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One gene directs both embryonic and adult stem cells to perform the self-renewal function that is crucial in their potential broad use in medical treatments, researchers said on Thursday.
   While the biology of these types of stem cells is very different, a study published in the journal Cell showed that they share at least this one key feature — a gene called Zfx that controls their ability to self-renew. 
 Stem cells are a kind of master cell for the body, capable of transforming into various tissue and cell types, offering hope that they can be used to repair tissue damaged by disease or injury.

ادامه مطلب
+ نوشته شده در  یکشنبه دوم اردیبهشت 1386ساعت 13:49  توسط پيمان كاظميان حدادي  | 

DNA fingerprinting begins by extracting DNA from the cells in a sample of blood, saliva, semen, or other appropriate fluid or tissue.

RFLP analysis

When DNA fingerprinting first began, restriction fragment length polymorphism (RFLP) analysis was used, though it has been almost completely replaced with newer techniques. RFLP analysis is performed by using a restriction enzyme to cut the DNA into fragments which are separated into bands during agarose gel electrophoresis. Next, the bands of DNA are transferred via a technique called Southern blotting from the agarose gel to a nylon membrane.

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ادامه مطلب
+ نوشته شده در  یکشنبه دوم اردیبهشت 1386ساعت 9:35  توسط پيمان كاظميان حدادي  | 

Genetic fingerprinting, DNA testing, DNA typing, and DNA profiling are techniques used to distinguish between individuals of the same species using only samples of their DNA. Its invention by Sir Alec Jeffreys at the University of Leicester was announced in 1985. Two humans will have the vast majority of their DNA sequence in common. Genetic fingerprinting exploits highly variable repeating sequences called minisatellites. Two unrelated humans will be likely to have different numbers of minisatellites at a given locus. In STR profiling, which is distinct from DNA fingerprinting, PCR is used to obtain enough DNA to then detect the number of repeats at several loci. It is possible to establish a match that is extremely unlikely to have arisen by coincidence, except in the case of identical twins, who will have identical genetic profiles.


ادامه مطلب
+ نوشته شده در  یکشنبه دوم اردیبهشت 1386ساعت 9:24  توسط پيمان كاظميان حدادي  |